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Palanivel, K. M.
- A Rare Clinical Occurrence of Canine Coccidiosis
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Authors
M. Geetha
1,
K. M. Palanivel
1
Affiliations
1 Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal (T.N.), IN
1 Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal (T.N.), IN
Source
Rashtriya Krishi (English), Vol 12, No 1 (2017), Pagination: 35-36Abstract
A male non descript one and half month old puppy was presented to infectious unit of Teaching Veterinary Clinical Complex, Veterinary College and Research Institute with a history of vomiting, black colour watery diarrhea. On physical examination, the animal had pyrexia, pale mucous membrane, tick infestation and severe dehydration with sunken eyeballs. The puppy passed blackish watery diarrhoeic faeces and raw blood at the end of defaecation. on faecal examination unsproulated oocysts of Isospora (Syn: Cystisospora) species were detected. Haemogram of the puppy revealed anaemia and neutrophilia. The pup was treated fluid therapy at the dose rate of 10 ml/kg body weight and haemtenics followed by with oriprim at the dose rate of 25 mg/ kg for five days.References
- Dubey, J.P. and Greene, C.E. (2012). Enteric coccidiosis, In. Greene, C.E. (Ed.,), Infectious diseases of dogs and cats, 4th Edn., Elsevers Saunders, 3251, Riverpost lane, St.Louis, Missouri, United States of America, pp. 828-839.
- Zajac, A.M. (1994). Faecal examination in the diagnosis of parasitism, In: Sloss, M.W., R.L.Kemp and A.M.Zajac, (Ed.,) Veterinary Clinical parasitology, 6th Ed., Iowa state university press, Ames, Iowa, pp.3-45.
- Development of Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Canine Leptospirosis
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Materials and Methods: An i-ELISA was developed using outer membrane protein extracted from Leptospira interrogans serovar canicola used for coating the well with concentration of 0.5 μg/μl. A total of 250 serum samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal, and subjected to i-ELISA.
Results: Out of 250 sera samples, 140 (56.00%) were found to be positive by i-ELISA. All the sera samples were subjected to microagglutination test (MAT) with panel of 12 different serovars. A total of 71 (28.40%) sera samples were positivity to MAT excluding the sera samples positive to L. interrogans serovars canicola and icterohaemorrhagiae in vaccinated dogs. Sensitivity and specificity of i-ELISA were higher in compared with MAT was 91.54% and 58.10%, respectively.
Conclusion: An indirect ELISA developed for the detection of canine antileptospiral antibodies proved to be highly sensitive, rapid and easy to perform and overcome the drawbacks of MAT.
Authors
Affiliations
1 Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal-637002, Tamil Nadu, IN
1 Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal-637002, Tamil Nadu, IN
Source
Veterinary World, Vol 10, No 5 (2017), Pagination: 530-535Abstract
Aim: This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against Leptospira spp. in canines.Materials and Methods: An i-ELISA was developed using outer membrane protein extracted from Leptospira interrogans serovar canicola used for coating the well with concentration of 0.5 μg/μl. A total of 250 serum samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal, and subjected to i-ELISA.
Results: Out of 250 sera samples, 140 (56.00%) were found to be positive by i-ELISA. All the sera samples were subjected to microagglutination test (MAT) with panel of 12 different serovars. A total of 71 (28.40%) sera samples were positivity to MAT excluding the sera samples positive to L. interrogans serovars canicola and icterohaemorrhagiae in vaccinated dogs. Sensitivity and specificity of i-ELISA were higher in compared with MAT was 91.54% and 58.10%, respectively.
Conclusion: An indirect ELISA developed for the detection of canine antileptospiral antibodies proved to be highly sensitive, rapid and easy to perform and overcome the drawbacks of MAT.